National usb microscope driver9/2/2023 elegans ( Rieckher et al., 2015), Drosophila ( Keller et al., 2010) and zebrafish ( Kobitski et al., 2015 Wan et al., 2019), more recently it has also found its use in functional imaging as a complement to already established imaging methods such as two photon imaging. While SPIM imaging has been originally used for the study of developmental processes in a number of animal models including embryos of C. Rapid movement of the sheet through the tissue then allows for volumetric recordings with cellular resolution. This is achieved by excitation of fluorescent structural or functional reporters expressed in the sample by a few micron thick light sheet and simultaneous recording by an orthogonally positioned high-resolution camera focused on the plane of excitation. ![]() Selective Plane Illumination Microscopy (SPIM) is a powerful method for 4D imaging of biological samples at a high spatio-temporal resolution ( Ahrens et al., 2013 Power and Huisken, 2017).
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